Cysteine ELISA Kit

Cysteine (abbreviated as Cys or C) is an alpha-amino acid with the chemical formula HO2CCH (NH2) CH2SH. It is a semi-essential amino acid, which means that it can be biosynthesized in humans. Its codons are UGU and UGC. Cysteine was considered to be a hydrophilic amino acid based on the belief that the thiol group interacts well with water. However, the cysteine side chain participates in the hydrophobic bonding system of the micelle. The quantitative comparison of the values of the critical micelle concentrations leads to the conclusion that the CH2SH group of amino acid has the same effect on the micelle stability as a methylene group of the hydrocarbon tail. Therefore, there is justification for the recently voiced assumptions of the hydrophobic nature of the cysteine side chain in proteins. The thiol side chain often participates in enzymatic reactions, serving as a nucleophile. The thiol is susceptible to oxidization to give the disulfide derivative cystine, which serves an important structural role in many proteins. When used as a food additive, it has the E-number E920.

Aviva Systems Biology Cysteine ELISA Kit (OKEH02584) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Cysteine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cysteine and incubated. The Cysteine found in the sample or standards competes with the biotinylated Cysteine for limited binding sites on the immobilized anti-Cysteine antibody. Excess unbound biotinylated Cysteine and sample or standard Cysteine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cysteine captured in the well and inversely proportional to the amount of Cysteine which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol