Noradrenaline ELISA Kit

One of the most important functions of Noradrenaline is its role as the neurotransmitter released from the sympathetic neurons affecting the heart. An increase in Noradrenaline from the sympathetic nervous system increases the rate of contractions. When Noradrenaline acts as a drug, it increases blood pressure by increasing vascular tone (tension of vascular smooth muscle) through alpha-adrenergic receptor activation; a reflex bradycardia homeostatic baroreflex is overcome by a compensatory reflex preventing an otherwise inevitable drop in heart rate to maintain blood pressure. Noradrenaline is synthesized from dopamine by dopamine beta-hydroxylase.[8] It is released from the adrenal medulla into the blood as a hormone, and is also a neurotransmitter in the central nervous system and sympathetic nervous system, where it is released from noradrenergic neurons in the locus coeruleus. The actions of norepinephrine are carried out via the binding to adrenergic receptors.

Aviva Systems Biology Noradrenaline ELISA Kit (OKEH02565) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Noradrenaline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Noradrenaline and incubated. The Noradrenaline found in the sample or standards competes with the biotinylated Noradrenaline for limited binding sites on the immobilized anti-Noradrenaline antibody. Excess unbound biotinylated Noradrenaline and sample or standard Noradrenaline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Noradrenaline captured in the well and inversely proportional to the amount of Noradrenaline which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol