What is the catalog number for Endotoxin ELISA Kit?

The catalog number for Endotoxin ELISA Kit is OKEH02559-96W.

What sizes are available for Endotoxin ELISA Kit?

Endotoxin ELISA Kit is available in the following size(s): 96 Well.

Is Endotoxin ELISA Kit in stock?

Endotoxin ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

Endotoxin ELISA Kit

Product Specifications

CAS Number

7732-18-5

Gene Aliases

ET, Endotoxin, ET

Reactivity

All Species

Target

Endotoxins are a component of the cell wall of certain bacteria. Bacteria which contain endotoxin are known as gram negative bacteria. The endotoxins are released from the bacteria as they are destroyed and elicit a strong response from the immune system by activating various components of the immune system and inducing cytokine release.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Endotoxin ELISA Kit (OKEH02559) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Endotoxin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Endotoxin and incubated. The Endotoxin found in the sample or standards competes with the biotinylated Endotoxin for limited binding sites on the immobilized anti-Endotoxin antibody. Excess unbound biotinylated Endotoxin and sample or standard Endotoxin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Endotoxin captured in the well and inversely proportional to the amount of Endotoxin which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol