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F13B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Coagulation factor XIII B chain

Gene Aliases

Coagulation factor XIII B chain; coagulation factor XIII, B polypeptide; fibrin-stabilizing factor B subunit; FXIIIB; protein-glutamine gamma-glutamyltransferase B chain; TGase; transglutaminase B chain.

Gene ID

2165

Accession Number

NP_001985.2

Reactivity

Homo sapiens, Human

Target

Coagulation factor XIII B chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology F13B ELISA Kit (Human) (OKEH02523) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F13B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F13B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F13B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.807 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

76 kDa

Specificity

Natural and recombinant Human Coagulation factor XIII B chain

Protein Length

661

NCBI Gene Symbol

F13B

Protein Name

P05160

Gene Name URL

F13B

Nucleotide Accession Number

NM_001994.2

Frequently Asked Questions

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