What is the catalog number for HMBS ELISA Kit (Human)?

The catalog number for HMBS ELISA Kit (Human) is OKEH02521-96W.

What sizes are available for HMBS ELISA Kit (Human)?

HMBS ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is HMBS ELISA Kit (Human) in stock?

HMBS ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

HMBS ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Hydroxymethylbilane synthase

Gene Aliases

Hydroxymethylbilane synthase; PBGD; PBG-D; PORC; porphobilinogen deaminase; porphyria, acute; Chester type; pre-uroporphyrinogen synthase; UPS; uroporphyrinogen I synthase; uroporphyrinogen I synthetase.

Gene ID

3145

Accession Number

NP_000181.2

Reactivity

Homo sapiens, Human

Target

Porphobilinogen deaminase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HMBS ELISA Kit (Human) (OKEH02521) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human HMBS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HMBS and incubated. The HMBS found in the sample or standards competes with the biotinylated HMBS for limited binding sites on the immobilized anti-Human HMBS antibody. Excess unbound biotinylated HMBS and sample or standard HMBS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HMBS captured in the well and inversely proportional to the amount of HMBS which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol