Gm1673 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Predicted gene 1673
Gene Aliases
Neuropeptide-like protein C4orf48 homolog.
Gene ID
381633
Accession Number
NP_001028630.2
Reactivity
Mouse, Mus musculus
Target
Neuropeptide-like protein C4orf48 homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Gm1673 ELISA Kit (Mouse) (OKEH02514) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Gm1673 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gm1673 and incubated. The Gm1673 found in the sample or standards competes with the biotinylated Gm1673 for limited binding sites on the immobilized anti-Mouse Gm1673 antibody. Excess unbound biotinylated Gm1673 and sample or standard Gm1673 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gm1673 captured in the well and inversely proportional to the amount of Gm1673 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
9 kDa
Specificity
Natural and recombinant Mouse Neuropeptide-like protein C4orf48 homolog
Protein Length
90
NCBI Gene Symbol
Gm1673
Protein Name
Q3UR78
Gene Name URL
Gm1673
Nucleotide Accession Number
NM_001033458.4
Frequently Asked Questions
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