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Gm1673 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Predicted gene 1673

Gene Aliases

Neuropeptide-like protein C4orf48 homolog.

Gene ID

381633

Accession Number

NP_001028630.2

Reactivity

Mouse, Mus musculus

Target

Neuropeptide-like protein C4orf48 homolog

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Gm1673 ELISA Kit (Mouse) (OKEH02514) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Gm1673 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gm1673 and incubated. The Gm1673 found in the sample or standards competes with the biotinylated Gm1673 for limited binding sites on the immobilized anti-Mouse Gm1673 antibody. Excess unbound biotinylated Gm1673 and sample or standard Gm1673 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gm1673 captured in the well and inversely proportional to the amount of Gm1673 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

0.39 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

9 kDa

Specificity

Natural and recombinant Mouse Neuropeptide-like protein C4orf48 homolog

Protein Length

90

NCBI Gene Symbol

Gm1673

Protein Name

Q3UR78

Gene Name URL

Gm1673

Nucleotide Accession Number

NM_001033458.4

Frequently Asked Questions

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