MPEG1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Macrophage expressed 1
Gene Aliases
IMD77; macrophage expressed gene 1; macrophage gene 1 protein; macrophage-expressed gene 1 protein; Mpg-1; MPG1; MPS1; P-2; perforin 2.
Gene ID
219972
Accession Number
NP_001034485.1
Reactivity
Homo sapiens, Human
Target
Macrophage-expressed gene 1 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MPEG1 ELISA Kit (Human) (OKEH02510) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human MPEG1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MPEG1 and incubated. The MPEG1 found in the sample or standards competes with the biotinylated MPEG1 for limited binding sites on the immobilized anti-Human MPEG1 antibody. Excess unbound biotinylated MPEG1 and sample or standard MPEG1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MPEG1 captured in the well and inversely proportional to the amount of MPEG1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
Sensitivity
0.072 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Human Macrophage-expressed gene 1 protein
Protein Length
716
NCBI Gene Symbol
MPEG1
Protein Name
Q2M385
Gene Name URL
MPEG1
Nucleotide Accession Number
NM_001039396.1
Frequently Asked Questions
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