VIPR2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Vasoactive intestinal peptide receptor 2
Gene Aliases
C16DUPq36.3; DUP7q36.3; helodermin-preferring VIP receptor; PACAP type III receptor; PACAP-R3; PACAP-R-3; pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal polypeptide receptor 2; VIP and PACAP receptor 2; VIP-R-2; VPAC2; VPAC2R; VPCAP2R.
Gene ID
7434
Accession Number
NP_001291451.1
Reactivity
Homo sapiens, Human
Target
Vasoactive intestinal polypeptide receptor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology VIPR2 ELISA Kit (Human) (OKEH02502) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VIPR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VIPR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VIPR2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
49 kDa
Specificity
Natural and recombinant Human Vasoactive intestinal polypeptide receptor 2
Protein Length
438
NCBI Gene Symbol
VIPR2
Protein Name
P41587
Gene Name URL
VIPR2
Nucleotide Accession Number
NM_001304522.1
Frequently Asked Questions
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