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ADCYAP1R1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

ADCYAP receptor type I

Gene Aliases

Adenylate cyclase activating polypeptide 1 (pituitary) receptor type I; PAC1; PAC1R; PACAP receptor 1; PACAP type I receptor; PACAPR; PACAP-R1; PACAPRI; pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop; pituitary adenylate cyclase-activating polypeptide type 1 receptor; pituitary adenylate cyclase-activating polypeptide type I receptor.

Gene ID

117

Accession Number

NP_001109.2

Reactivity

Homo sapiens, Human

Target

Pituitary adenylate cyclase-activating polypeptide type I receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ADCYAP1R1 ELISA Kit (Human) (OKEH02500) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADCYAP1R1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADCYAP1R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADCYAP1R1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

53 kDa

Specificity

Natural and recombinant Human Pituitary adenylate cyclase-activating polypeptide type I receptor

Protein Length

468

NCBI Gene Symbol

ADCYAP1R1

Protein Name

P41586

Gene Name URL

ADCYAP1R1

Nucleotide Accession Number

NM_001118.4

Frequently Asked Questions

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