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SERPINA4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Serpin family A member 4

Gene Aliases

KAL; kallikrein inhibitor; kallistatin; KLST; KST; peptidase inhibitor 4; PI4; PI-4; protease inhibitor 4 (kallistatin) ; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4; serpin A4; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4; sserpin family A member 4; testicular secretory protein Li 22.

Gene ID

5267

Accession Number

NP_001275961.1

Reactivity

Homo sapiens, Human

Target

Kallistatin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SERPINA4 ELISA Kit (Human) (OKEH02499) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SERPINA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 kDa

Specificity

Natural and recombinant Human Kallistatin

Protein Length

427

NCBI Gene Symbol

SERPINA4

Protein Name

P29622

Gene Name URL

SERPINA4

Nucleotide Accession Number

NM_001289032.1

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