LGALS3BP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Galectin 3 binding protein

Gene Aliases

90K; basement membrane autoantigen p105; BTBD17B; CyCAP; galectin-3-binding protein; gp90; L3 antigen; lectin galactoside-binding soluble 3-binding protein; M2BP; mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2-BP; serum protein 90K; TANGO10B; transport and golgi organization 10 homolog B; tumor-associated antigen 90K.

Gene ID

3959

Accession Number

NP_005558.1

Reactivity

Homo sapiens, Human

Target

Galectin-3-binding protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LGALS3BP ELISA Kit (Human) (OKEH02493) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGALS3BP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGALS3BP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGALS3BP captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol