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CDK5R1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cyclin dependent kinase 5 regulatory subunit 1

Gene Aliases

CDK5 activator 1; CDK5P35; CDK5R; cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; NCK5A; neuronal CDK5 activator; p23; p25; p35; p35nck5a; regulatory partner for CDK5 kinase; tau protein kinase II 23kDa subunit; TPKII regulatory subunit.

Gene ID

8851

Accession Number

NP_003876.1

Reactivity

Homo sapiens, Human

Target

Cyclin-dependent kinase 5 activator 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CDK5R1 ELISA Kit (Human) (OKEH02489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK5R1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK5R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK5R1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

31 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 kDa

Specificity

Natural and recombinant Human Cyclin-dependent kinase 5 activator 1

Protein Length

307

NCBI Gene Symbol

CDK5R1

Protein Name

Q15078

Gene Name URL

CDK5R1

Nucleotide Accession Number

NM_003885.2

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