RET ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Ret proto-oncogene
Gene Aliases
Cadherin family member 12; cadherin-related family member 16; CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; proto-oncogene c-Ret; proto-oncogene tyrosine-protein kinase receptor Ret; PTC; rearranged during transfection; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease) ; RET receptor tyrosine kinase; RET-ELE1.
Gene ID
5979
Accession Number
NP_065681.1
Reactivity
Homo sapiens, Human
Target
Proto-oncogene tyrosine-protein kinase receptor Ret
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RET ELISA Kit (Human) (OKEH02483) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RET has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RET is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RET captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
15 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
124 kDa
Specificity
Natural and recombinant Human Proto-oncogene tyrosine-protein kinase receptor Ret
Protein Length
1114
NCBI Gene Symbol
RET
Protein Name
P07949
Gene Name URL
RET
Nucleotide Accession Number
NM_020630.4
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