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RET ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Ret proto-oncogene

Gene Aliases

Cadherin family member 12; cadherin-related family member 16; CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; proto-oncogene c-Ret; proto-oncogene tyrosine-protein kinase receptor Ret; PTC; rearranged during transfection; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease) ; RET receptor tyrosine kinase; RET-ELE1.

Gene ID

5979

Accession Number

NP_065681.1

Reactivity

Homo sapiens, Human

Target

Proto-oncogene tyrosine-protein kinase receptor Ret

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RET ELISA Kit (Human) (OKEH02483) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RET has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RET is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RET captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

15 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

124 kDa

Specificity

Natural and recombinant Human Proto-oncogene tyrosine-protein kinase receptor Ret

Protein Length

1114

NCBI Gene Symbol

RET

Protein Name

P07949

Gene Name URL

RET

Nucleotide Accession Number

NM_020630.4

Frequently Asked Questions

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