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BAD ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

BCL2 associated agonist of cell death

Gene Aliases

BBC2; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; bcl2-associated agonist of cell death; bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; bcl2-L-8; BCL2L8; bcl-2-like protein 8; BCL-X/BCL-2 binding protein; bcl-XL/Bcl-2-associated death promoter.

Gene ID

572

Accession Number

NP_004313.1

Reactivity

Homo sapiens, Human

Target

Bcl2-associated agonist of cell death

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BAD ELISA Kit (Human) (OKEH02462) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAD captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 kDa

Specificity

Natural and recombinant Human Bcl2-associated agonist of cell death

Protein Length

168

NCBI Gene Symbol

BAD

Protein Name

Q92934

Gene Name URL

BAD

Nucleotide Accession Number

NM_004322.3

Frequently Asked Questions

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