HLA-DRB5 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Major histocompatibility complex, class II, DR beta 5
Gene Aliases
DR beta-5; DR2-beta-2; Dw2; HLA class II histocompatibility antigen, DR-5 beta chain; HLA-DRB5*; major histocompatibility complex, class II, DR beta 5; MHC class II antigen DRB5.
Gene ID
3127
Accession Number
NP_002116.2
Reactivity
Homo sapiens, Human
Target
HLA class II histocompatibility antigen, DR beta 5 chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology HLA-DRB5 ELISA Kit (Human) (OKEH02460) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human HLA-DRB5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HLA-DRB5 and incubated. The HLA-DRB5 found in the sample or standards competes with the biotinylated HLA-DRB5 for limited binding sites on the immobilized anti-Human HLA-DRB5 antibody. Excess unbound biotinylated HLA-DRB5 and sample or standard HLA-DRB5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HLA-DRB5 captured in the well and inversely proportional to the amount of HLA-DRB5 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
30 kDa
Specificity
Natural and recombinant Human HLA class II histocompatibility antigen, DR beta 5 chain
Protein Length
266
NCBI Gene Symbol
HLA-DRB5
Protein Name
Q30154
Gene Name URL
HLA-DRB5
Nucleotide Accession Number
NM_002125.3
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