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CNP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

2',3'-cyclic nucleotide 3' phosphodiesterase

Gene Aliases

2', 3' cyclic nucleotide 3' phosphohydrolase;2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1; CNPase; HLD20.

Gene ID

1267

Accession Number

NP_149124.3

Reactivity

Homo sapiens, Human

Target

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CNP ELISA Kit (Human) (OKEH02453) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human CNP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CNP and incubated. The CNP found in the sample or standards competes with the biotinylated CNP for limited binding sites on the immobilized anti-Human CNP antibody. Excess unbound biotinylated CNP and sample or standard CNP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CNP captured in the well and inversely proportional to the amount of CNP which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.15 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

48 kDa

Specificity

Natural and recombinant Human 2',3'-cyclic-nucleotide 3'-phosphodiesterase

Protein Length

421

NCBI Gene Symbol

CNP

Protein Name

P09543

Gene Name URL

CNP

Nucleotide Accession Number

NM_033133.4

Frequently Asked Questions

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