TTPAL ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Alpha tocopherol transfer protein like
Gene Aliases
Alpha-tocopherol transfer protein-like; C20orf121; tocopherol (alpha) transfer protein-like.
Gene ID
79183
Accession Number
NP_001034288.1
Reactivity
Homo sapiens, Human
Target
Alpha-tocopherol transfer protein-like
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TTPAL ELISA Kit (Human) (OKEH02432) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TTPAL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TTPAL and incubated. The TTPAL found in the sample or standards competes with the biotinylated TTPAL for limited binding sites on the immobilized anti-Human TTPAL antibody. Excess unbound biotinylated TTPAL and sample or standard TTPAL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TTPAL captured in the well and inversely proportional to the amount of TTPAL which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Human Alpha-tocopherol transfer protein-like
Protein Length
342
NCBI Gene Symbol
TTPAL
Protein Name
Q9BTX7
Gene Name URL
TTPAL
Nucleotide Accession Number
NM_001039199.2
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