SERPINB13 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serpin family B member 13
Gene Aliases
HaCaT UV-repressible serpin; headpin; HSHUR7SEQ; HUR7; HURPIN; peptidase inhibitor 13; PI13; PI-13; protease inhibitor 13 (hurpin, headpin) ; proteinase inhibitor 13; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13; serpin B13; serpin peptidase inhibitor, clade B (ovalbumin), member 13; UV-B repressed sequence, HUR 7.
Gene ID
5275
Accession Number
NP_001294852.1
Reactivity
Homo sapiens, Human
Target
Serpin B13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SERPINB13 ELISA Kit (Human) (OKEH02431) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human SERPINB13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINB13 and incubated. The SERPINB13 found in the sample or standards competes with the biotinylated SERPINB13 for limited binding sites on the immobilized anti-Human SERPINB13 antibody. Excess unbound biotinylated SERPINB13 and sample or standard SERPINB13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINB13 captured in the well and inversely proportional to the amount of SERPINB13 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
44 kDa
Specificity
Natural and recombinant Human Serpin B13
Protein Length
391
NCBI Gene Symbol
SERPINB13
Protein Name
Q9UIV8
Gene Name URL
SERPINB13
Nucleotide Accession Number
NM_001307923.1
Frequently Asked Questions
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