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SERPINB13 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Serpin family B member 13

Gene Aliases

HaCaT UV-repressible serpin; headpin; HSHUR7SEQ; HUR7; HURPIN; peptidase inhibitor 13; PI13; PI-13; protease inhibitor 13 (hurpin, headpin) ; proteinase inhibitor 13; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13; serpin B13; serpin peptidase inhibitor, clade B (ovalbumin), member 13; UV-B repressed sequence, HUR 7.

Gene ID

5275

Accession Number

NP_001294852.1

Reactivity

Homo sapiens, Human

Target

Serpin B13

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SERPINB13 ELISA Kit (Human) (OKEH02431) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human SERPINB13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINB13 and incubated. The SERPINB13 found in the sample or standards competes with the biotinylated SERPINB13 for limited binding sites on the immobilized anti-Human SERPINB13 antibody. Excess unbound biotinylated SERPINB13 and sample or standard SERPINB13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINB13 captured in the well and inversely proportional to the amount of SERPINB13 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

44 kDa

Specificity

Natural and recombinant Human Serpin B13

Protein Length

391

NCBI Gene Symbol

SERPINB13

Protein Name

Q9UIV8

Gene Name URL

SERPINB13

Nucleotide Accession Number

NM_001307923.1

Frequently Asked Questions

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