RAB19 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
RAB19, member RAS oncogene family
Gene Aliases
Ras-related protein Rab-19.
Gene ID
19331
Accession Number
NP_035356.1
Reactivity
Mouse, Mus musculus
Target
Ras-related protein Rab-19
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology RAB19 ELISA Kit (Mouse) (OKEH02420) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse RAB19 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RAB19 and incubated. The RAB19 found in the sample or standards competes with the biotinylated RAB19 for limited binding sites on the immobilized anti-Mouse RAB19 antibody. Excess unbound biotinylated RAB19 and sample or standard RAB19 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RAB19 captured in the well and inversely proportional to the amount of RAB19 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
24 kDa
Specificity
Natural and recombinant Mouse Ras-related protein Rab-19
Protein Length
217
NCBI Gene Symbol
Rab19
Protein Name
P35294
Gene Name URL
Rab19
Nucleotide Accession Number
NM_011226.1
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