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RAB14 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

RAB14, member RAS oncogene family

Gene Aliases

BA165P4.3 (member RAS oncogene family) ; F protein-binding protein 1; FBP; RAB-14; ras-related protein Rab-14; small GTP binding protein RAB14.

Gene ID

51552

Accession Number

NP_057406.2

Reactivity

Homo sapiens, Human

Target

Ras-related protein Rab-14

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RAB14 ELISA Kit (Human) (OKEH02419) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAB14 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

0.18 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

24 kDa

Specificity

Natural and recombinant Human Ras-related protein Rab-14

Protein Length

215

NCBI Gene Symbol

RAB14

Protein Name

P61106

Gene Name URL

RAB14

Nucleotide Accession Number

NM_016322.3

Frequently Asked Questions

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