MUP20 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Major urinary protein 20
Gene Aliases
Da; darcin; Gm12560; major urinary protein 20; major urinary protein 24; Mup24.
Gene ID
381530
Accession Number
NP_001012323.1
Reactivity
Mouse, Mus musculus
Target
Major urinary protein 20
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MUP20 ELISA Kit (Mouse) (OKEH02418) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MUP20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUP20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MUP20 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 103%
Sensitivity
0.186 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Mouse Major urinary protein 20
Protein Length
181
NCBI Gene Symbol
Mup20
Protein Name
Q5FW60
Gene Name URL
Mup20
Nucleotide Accession Number
NM_001012323.1
Frequently Asked Questions
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