What is the catalog number for HARS ELISA Kit (Human)?

The catalog number for HARS ELISA Kit (Human) is OKEH02410-96W.

What sizes are available for HARS ELISA Kit (Human)?

HARS ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is HARS ELISA Kit (Human) in stock?

HARS ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

HARS ELISA Kit (Human)

Selected Variant

OKEH02410-96W

Size: 96 Tests

Product Specifications

CAS Number

7732-18-5

Gene Name

Histidyl-tRNA synthetase 1

Gene Aliases

CMT2W; HARS; HisRS; histidine translase; histidine--tRNA ligase, cytoplasmic; Histidyl-tRNA synthetase; HRS; Jo-1 antigen; USH3B.

Gene ID

3035

Accession Number

NP_001244969.1

Reactivity

Homo sapiens, Human

Target

Histidine--tRNA ligase, cytoplasmic

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HARS ELISA Kit (Human) (OKEH02410) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HARS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HARS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HARS captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

57 kDa

Specificity

Natural and recombinant Human Histidine--tRNA ligase, cytoplasmic

Protein Length

509

NCBI Gene Symbol

HARS1

Protein Name

P12081

Gene Name URL

HARS1

Nucleotide Accession Number

NM_001258040.2

Price

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