What is the catalog number for AMFR ELISA Kit (Human)?

The catalog number for AMFR ELISA Kit (Human) is OKEH02407-96W.

What sizes are available for AMFR ELISA Kit (Human)?

AMFR ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is AMFR ELISA Kit (Human) in stock?

AMFR ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

AMFR ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Autocrine motility factor receptor

Gene Aliases

Autocrine motility factor receptor; E3 ubiquitin-protein ligase AMFR; GP78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR; RNF45.

Gene ID

267

Accession Number

NP_001135.3

Reactivity

Homo sapiens, Human

Target

E3 ubiquitin-protein ligase AMFR

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AMFR ELISA Kit (Human) (OKEH02407) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AMFR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AMFR and incubated. The AMFR found in the sample or standards competes with the biotinylated AMFR for limited binding sites on the immobilized anti-Human AMFR antibody. Excess unbound biotinylated AMFR and sample or standard AMFR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AMFR captured in the well and inversely proportional to the amount of AMFR which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol