What is the catalog number for ZEB1 ELISA Kit (Human)?

The catalog number for ZEB1 ELISA Kit (Human) is OKEH02404-96W.

What sizes are available for ZEB1 ELISA Kit (Human)?

ZEB1 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is ZEB1 ELISA Kit (Human) in stock?

ZEB1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

ZEB1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Zinc finger E-box binding homeobox 1

Gene Aliases

AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; negative regulator of IL2; NIL2A; NIL-2-A zinc finger protein; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression) ; ZFHEP; ZFHX1A; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein.

Gene ID

6935

Accession Number

NP_001121600.1

Reactivity

Homo sapiens, Human

Target

Zinc finger E-box-binding homeobox 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZEB1 ELISA Kit (Human) (OKEH02404) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human ZEB1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZEB1 and incubated. The ZEB1 found in the sample or standards competes with the biotinylated ZEB1 for limited binding sites on the immobilized anti-Human ZEB1 antibody. Excess unbound biotinylated ZEB1 and sample or standard ZEB1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZEB1 captured in the well and inversely proportional to the amount of ZEB1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol