ISG15 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
ISG15 ubiquitin like modifier
Gene Aliases
G1P2; hUCRP; IFI15; IMD38; interferon, alpha-inducible protein (clone IFI-15K) ; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; IP17; ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP.
Gene ID
9636
Accession Number
NP_005092.1
Reactivity
Homo sapiens, Human
Target
Ubiquitin-like protein ISG15
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ISG15 ELISA Kit (Human) (OKEH02386) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ISG15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ISG15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ISG15 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.162 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 kDa
Notes
Formerly GWB-D2X4IV
Specificity
Natural and recombinant Human Ubiquitin-like protein ISG15
Protein Length
165
NCBI Gene Symbol
ISG15
Protein Name
P05161
Gene Name URL
ISG15
Nucleotide Accession Number
NM_005101.3
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