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DDX42 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

DEAD-box helicase 42

Gene Aliases

ATP-dependent RNA helicase DDX42; DDX42P; DEAD (Asp-Glu-Ala-Asp) box helicase 42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; SF3b125; SF3B8; splicing factor 3b, subunit 8; splicing factor 3B-associated 125 kDa protein.

Gene ID

11325

Accession Number

NP_031398.2

Reactivity

Homo sapiens, Human

Target

ATP-dependent RNA helicase DDX42

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DDX42 ELISA Kit (Human) (OKEH02374) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDX42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDX42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDX42 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

34 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

103 kDa

Specificity

Natural and recombinant Human ATP-dependent RNA helicase DDX42

Protein Length

938

NCBI Gene Symbol

DDX42

Protein Name

Q86XP3

Gene Name URL

DDX42

Nucleotide Accession Number

NM_007372.3

Frequently Asked Questions

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