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FAM3A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

FAM3 metabolism regulating signaling molecule A

Gene Aliases

2.19; cytokine-like protein 2-19; DLD; DXS560S; family with sequence similarity 3 member A; protein FAM3A; XAP-7.

Gene ID

60343

Accession Number

NP_001164603.1

Reactivity

Homo sapiens, Human

Target

Protein FAM3A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FAM3A ELISA Kit (Human) (OKEH02368) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAM3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAM3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAM3A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

25 kDa

Specificity

Natural and recombinant Human Protein FAM3A

Protein Length

230

NCBI Gene Symbol

FAM3A

Protein Name

P98173

Gene Name URL

FAM3A

Nucleotide Accession Number

NM_001171132.2

Frequently Asked Questions

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