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NDUFA6 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

NADH:ubiquinone oxidoreductase subunit A6

Gene Aliases

B14; CI-B14; Complex I-B14; LYR motif-containing protein 6; LYRM6; MC1DN33; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; NADHB14; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 6; NADH-ubiquinone oxidoreductase B14 subunit.

Gene ID

4700

Accession Number

NP_002481.2

Reactivity

Homo sapiens, Human

Target

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NDUFA6 ELISA Kit (Human) (OKEH02366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NDUFA6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NDUFA6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NDUFA6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.056 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 kDa

Specificity

Natural and recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Protein Length

154

NCBI Gene Symbol

NDUFA6

Protein Name

P56556

Gene Name URL

NDUFA6

Nucleotide Accession Number

NM_002490.3

Frequently Asked Questions

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