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ADAM28 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

ADAM metallopeptidase domain 28

Gene Aliases

ADAM 28; disintegrin and metalloproteinase domain-containing protein 28; eMDC II; eMDCII; epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymis secretory sperm binding protein; MDCL; MDC-L; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L.

Gene ID

10863

Accession Number

NP_001291280.1

Reactivity

Homo sapiens, Human

Target

Disintegrin and metalloproteinase domain-containing protein 28

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ADAM28 ELISA Kit (Human) (OKEH02363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM28 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM28 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM28 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

87 kDa

Specificity

Natural and recombinant Human Disintegrin and metalloproteinase domain-containing protein 28

Protein Length

775

NCBI Gene Symbol

ADAM28

Protein Name

Q9UKQ2

Gene Name URL

ADAM28

Nucleotide Accession Number

NM_001304351.1

Frequently Asked Questions

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