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C1QTNF1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

C1q and tumor necrosis factor related protein 1

Gene Aliases

1600017K21Rik; Adip; adiponectin p; complement C1q tumor necrosis factor-related protein 1; CTR; CTRP1; putative secreted protein ZSIG37; Zsig37.

Gene ID

56745

Accession Number

NP_001191058.1

Reactivity

Mouse, Mus musculus

Target

Complement C1q tumor necrosis factor-related protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C1QTNF1 ELISA Kit (Mouse) (OKEH02361) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QTNF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QTNF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1QTNF1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.178 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

32 kDa

Specificity

Natural and recombinant Mouse Complement C1q tumor necrosis factor-related protein 1

Protein Length

281

NCBI Gene Symbol

C1qtnf1

Protein Name

Q9QXP7

Gene Name URL

C1qtnf1

Nucleotide Accession Number

NM_001204129.1

Frequently Asked Questions

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