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SIK1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Salt inducible kinase 1, salt inducible kinase 1B (putative)

Gene Aliases

DEE30; MSK; myocardial SNF1-like kinase; probable serine/threonine-protein kinase SIK1B; Salt-inducible kinase 1; salt-inducible kinase 1B; salt-inducible protein kinase 1; serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK; SIK1; SIK-1; SIK1B; SNF1-like kinase; SNF1LK.

Gene ID

102724428, 150094

Accession Number

NP_001307572.1

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein kinase SIK1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SIK1 ELISA Kit (Human) (OKEH02354) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SIK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SIK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SIK1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

85 kDa

Specificity

Natural and recombinant Human Serine/threonine-protein kinase SIK1

Protein Length

783

NCBI Gene Symbol

LOC102724428, SIK1

Protein Name

P57059

Gene Name URL

LOC102724428, SIK1

Nucleotide Accession Number

NM_001320643.1

Frequently Asked Questions

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