PIK3CA ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Gene Aliases
6330412C24Rik; caP; caPI3K; p110; p110alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphoinositide-3-kinase catalytic alpha polypeptide; PI3Kalpha; PI3K-alpha; PI3-kinase subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA.
Gene ID
18706
Accession Number
NP_032865.2
Reactivity
Mouse, Mus musculus
Target
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PIK3CA ELISA Kit (Mouse) (OKEH02352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
124 kDa
Specificity
Natural and recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Protein Length
1068
NCBI Gene Symbol
Pik3ca
Protein Name
P42337
Gene Name URL
Pik3ca
Nucleotide Accession Number
NM_008839.2
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