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MNAT1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MNAT1 component of CDK activating kinase

Gene Aliases

CAP35; CDK7/cyclin-H assembly factor; CDK-activating kinase assembly factor MAT1; cyclin G1 interacting protein; Cyclin-G1-interacting protein; MAT1; Menage a trois; menage a trois 1 (CAK assembly factor) ; menage a trois homolog 1, cyclin H assembly factor; menage a trois-like protein 1 cyclin H assembly factor; MNAT CDK-activating kinase assembly factor 1; MNAT1, CDK activating kinase assembly factor; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; TFB3.

Gene ID

4331

Accession Number

NP_001171434.1

Reactivity

Homo sapiens, Human

Target

CDK-activating kinase assembly factor MAT1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MNAT1 ELISA Kit (Human) (OKEH02344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MNAT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MNAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MNAT1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

34 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Human CDK-activating kinase assembly factor MAT1

Protein Length

309

NCBI Gene Symbol

MNAT1

Protein Name

P51948

Gene Name URL

MNAT1

Nucleotide Accession Number

NM_001177963.1

Frequently Asked Questions

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