AKR1C1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Aldo-keto reductase family 1 member C1
Gene Aliases
20 alpha-hydroxysteroid dehydrogenase;20-ALPHA-HSD;20-alpha-hydroxysteroid dehydrogenase;2-ALPHA-HSD; aldo-keto reductase C; aldo-keto reductase family 1 member C1; C9; chlordecone reductase homolog HAKRC; DD1; DD1/DD2; DDH; DDH1; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha) -hydroxysteroid dehydrogenase; H-37; HAKRC; HBAB; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; MBAB; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase.
Gene ID
1645
Accession Number
NP_001344.2
Reactivity
Homo sapiens, Human
Target
Aldo-keto reductase family 1 member C1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AKR1C1 ELISA Kit (Human) (OKEH02337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKR1C1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKR1C1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKR1C1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Human Aldo-keto reductase family 1 member C1
Protein Length
323
NCBI Gene Symbol
AKR1C1
Protein Name
Q04828
Gene Name URL
AKR1C1
Nucleotide Accession Number
NM_001353.5
Frequently Asked Questions
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