CNN2 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Calponin 2

Gene Aliases

Calponin H2, smooth muscle; calponin-2; neutral calponin.

Gene ID

539019

Accession Number

NP_001030497.1

Reactivity

Bos taurus, Bovine

Target

Calponin-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CNN2 ELISA Kit (Bovine) (OKEH02330) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine CNN2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CNN2 and incubated. The CNN2 found in the sample or standards competes with the biotinylated CNN2 for limited binding sites on the immobilized anti-Bovine CNN2 antibody. Excess unbound biotinylated CNN2 and sample or standard CNN2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CNN2 captured in the well and inversely proportional to the amount of CNN2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol