CXADR ELISA Kit (Human)
Product Specifications
Gene Name
CXADR Ig-like cell adhesion molecule
Gene Aliases
46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; CAR4/6; coxsackie virus and adenovirus receptor; coxsackievirus and adenovirus receptor; coxsackievirus B-adenovirus receptor; CVB3-binding protein; HCAR; HCVADR.
Gene ID
1525
Accession Number
NP_001193992.1
Reactivity
Homo sapiens, Human
Target
Coxsackievirus and adenovirus receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CXADR ELISA Kit (Human) (OKEH02324) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXADR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXADR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXADR captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
34 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
10 kDa
Specificity
Natural and recombinant Human Coxsackievirus and adenovirus receptor
Protein Length
365
NCBI Gene Symbol
CXADR
Protein Name
P78310
Gene Name URL
CXADR
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001207063.1
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