GPA33 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glycoprotein A33
Gene Aliases
A33; cell surface A33 antigen; Glycoprotein A33; glycoprotein A33 (transmembrane) ; transmembrane glycoprotein A33.
Gene ID
10223
Accession Number
NP_005805.1
Reactivity
Homo sapiens, Human
Target
Cell surface A33 antigen
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GPA33 ELISA Kit (Human) (OKEH02316) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPA33 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPA33 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GPA33 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
36 kDa
Specificity
Natural and recombinant Human Cell surface A33 antigen
Protein Length
319
NCBI Gene Symbol
GPA33
Protein Name
Q99795
Gene Name URL
GPA33
Nucleotide Accession Number
NM_005814.2
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