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CD74 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

CD74 molecule

Gene Aliases

CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ; CD74 molecule, major histocompatibility complex, class II invariant chain; DHLAG; gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-GAMMA; II; MHC HLA-DR gamma chain; p33.

Gene ID

972

Accession Number

NP_001020329.1

Reactivity

Homo sapiens, Human

Target

HLA class II histocompatibility antigen gamma chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD74 ELISA Kit (Human) (OKEH02313) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD74 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD74 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD74 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 kDa

Specificity

Natural and recombinant Human HLA class II histocompatibility antigen gamma chain

Protein Length

296

NCBI Gene Symbol

CD74

Protein Name

P04233

Gene Name URL

CD74

Nucleotide Accession Number

NM_001025158.2

Frequently Asked Questions

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