ITIH4 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Inter-alpha-trypsin inhibitor heavy chain 4
Gene Aliases
GP120; H4P; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ; inter-alpha-inhibitor heavy chain 4; inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain family member 4; inter-alpha-trypsin inhibitor heavy chain H4; inter-alpha-trypsin inhibitor, heavy chain-like, 1; ITI heavy chain H4; ITI-HC4; ITIHL1; PK120; PK-120; Plasma kallikrein sensitive glycoprotein 120; plasma kallikrein-sensitive glycoprotein 120.
Gene ID
3700
Accession Number
NP_001159921.1
Reactivity
Homo sapiens, Human
Target
Inter-alpha-trypsin inhibitor heavy chain H4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ITIH4 ELISA Kit (Human) (OKEH02311) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITIH4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITIH4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITIH4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
103 kDa
Specificity
Natural and recombinant Human Inter-alpha-trypsin inhibitor heavy chain H4
Protein Length
930
NCBI Gene Symbol
ITIH4
Protein Name
Q14624
Gene Name URL
ITIH4
Nucleotide Accession Number
NM_001166449.1
Frequently Asked Questions
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