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ITIH4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Inter-alpha-trypsin inhibitor heavy chain 4

Gene Aliases

GP120; H4P; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ; inter-alpha-inhibitor heavy chain 4; inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain family member 4; inter-alpha-trypsin inhibitor heavy chain H4; inter-alpha-trypsin inhibitor, heavy chain-like, 1; ITI heavy chain H4; ITI-HC4; ITIHL1; PK120; PK-120; Plasma kallikrein sensitive glycoprotein 120; plasma kallikrein-sensitive glycoprotein 120.

Gene ID

3700

Accession Number

NP_001159921.1

Reactivity

Homo sapiens, Human

Target

Inter-alpha-trypsin inhibitor heavy chain H4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ITIH4 ELISA Kit (Human) (OKEH02311) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITIH4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITIH4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITIH4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

103 kDa

Specificity

Natural and recombinant Human Inter-alpha-trypsin inhibitor heavy chain H4

Protein Length

930

NCBI Gene Symbol

ITIH4

Protein Name

Q14624

Gene Name URL

ITIH4

Nucleotide Accession Number

NM_001166449.1

Frequently Asked Questions

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