LMX1B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

LIM homeobox transcription factor 1 beta

Gene Aliases

FSGS10; LIM homeobox transcription factor 1-beta; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B; LMX1.2; LMX-1.2; NPS1.

Gene ID

4010

Accession Number

NP_001167618.1

Reactivity

Homo sapiens, Human

Target

LIM homeobox transcription factor 1-beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology LMX1B ELISA Kit (Human) (OKEH02309) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LMX1B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated LMX1B and incubated. The LMX1B found in the sample or standards competes with the biotinylated LMX1B for limited binding sites on the immobilized anti-Human LMX1B antibody. Excess unbound biotinylated LMX1B and sample or standard LMX1B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated LMX1B captured in the well and inversely proportional to the amount of LMX1B which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol