What is the catalog number for TPD52 ELISA Kit (Human)?

The catalog number for TPD52 ELISA Kit (Human) is OKEH02283-96W.

What sizes are available for TPD52 ELISA Kit (Human)?

TPD52 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TPD52 ELISA Kit (Human) in stock?

TPD52 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

TPD52 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Tumor protein D52

Gene Aliases

D52; hD52; N8L; PC-1; PrLZ; prostate and colon associated protein; prostate leucine zipper; protein N8; tumor protein D52.

Gene ID

7163

Accession Number

NP_001020423.1

Reactivity

Homo sapiens, Human

Target

Tumor protein D52

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology TPD52 ELISA Kit (Human) (OKEH02283) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TPD52 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TPD52 and incubated. The TPD52 found in the sample or standards competes with the biotinylated TPD52 for limited binding sites on the immobilized anti-Human TPD52 antibody. Excess unbound biotinylated TPD52 and sample or standard TPD52 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TPD52 captured in the well and inversely proportional to the amount of TPD52 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol