CD300LB ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
CD300 molecule like family member b
Gene Aliases
CD300 antigen like family member B; CD300 antigen-like family member B; CD300b; CLM7; CLM-7; CMRF35-A2; CMRF35-like molecule 7; immune receptor expressed on myeloid cells 3; IREM3; IREM-3; leukocyte mono-Ig-like receptor 5; TREM5; TREM-5; triggering receptor expressed on myeloid cells 5.
Gene ID
124599
Accession Number
NP_777552.3
Reactivity
Homo sapiens, Human
Target
CMRF35-like molecule 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD300LB ELISA Kit (Human) (OKEH02265) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD300LB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD300LB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD300LB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
23 kDa
Specificity
Natural and recombinant Human CMRF35-like molecule 7
Protein Length
201
NCBI Gene Symbol
CD300LB
Protein Name
A8K4G0
Gene Name URL
CD300LB
Nucleotide Accession Number
NM_174892.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog