SLC30A3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Solute carrier family 30 member 3
Gene Aliases
Solute carrier family 30 (zinc transporter), member 3; Solute carrier family 30 member 3; zinc transporter 3; zinc transporter ZnT-3; znT-3; ZNT3.
Gene ID
7781
Accession Number
NP_001305878.1
Reactivity
Homo sapiens, Human
Target
Zinc transporter 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology SLC30A3 ELISA Kit (Human) (OKEH02261) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human SLC30A3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC30A3 and incubated. The SLC30A3 found in the sample or standards competes with the biotinylated SLC30A3 for limited binding sites on the immobilized anti-Human SLC30A3 antibody. Excess unbound biotinylated SLC30A3 and sample or standard SLC30A3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC30A3 captured in the well and inversely proportional to the amount of SLC30A3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Zinc transporter 3
Protein Length
388
NCBI Gene Symbol
SLC30A3
Protein Name
Q99726
Gene Name URL
SLC30A3
Nucleotide Accession Number
NM_001318949.1
Frequently Asked Questions
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