DPP9 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Dipeptidyl peptidase 9
Gene Aliases
Dipeptidyl peptidase 9; Dipeptidyl peptidase IV-related protein 2; dipeptidyl peptidase IV-related protein-2; dipeptidyl peptidase IX; dipeptidyl peptidase-like protein 9; DP9; DPLP9; DPP IX; DPRP2; DPRP-2.
Gene ID
91039
Accession Number
NP_631898.3
Reactivity
Homo sapiens, Human
Target
Dipeptidyl peptidase 9
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DPP9 ELISA Kit (Human) (OKEH02253) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPP9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPP9 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
Sensitivity
0.43 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
98 kDa
Specificity
Natural and recombinant Human Dipeptidyl peptidase 9
Protein Length
863
NCBI Gene Symbol
DPP9
Protein Name
Q86TI2
Gene Name URL
DPP9
Nucleotide Accession Number
NM_139159.4
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