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MTRNR2L6 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MT-RNR2 like 6

Gene Aliases

HN6; humanin-like 6; humanin-like protein 6; MTRNR2-like 6; MT-RNR2-like protein 6.

Gene ID

100463482

Accession Number

NP_001177416.1

Reactivity

Homo sapiens, Human

Target

Humanin-like 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology MTRNR2L6 ELISA Kit (Human) (OKEH02250) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human MTRNR2L6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MTRNR2L6 and incubated. The MTRNR2L6 found in the sample or standards competes with the biotinylated MTRNR2L6 for limited binding sites on the immobilized anti-Human MTRNR2L6 antibody. Excess unbound biotinylated MTRNR2L6 and sample or standard MTRNR2L6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MTRNR2L6 captured in the well and inversely proportional to the amount of MTRNR2L6 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.022 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

2 kDa

Specificity

Natural and recombinant Human Humanin-like 6

Protein Length

24

NCBI Gene Symbol

MTRNR2L6

Protein Name

P0CJ73

Gene Name URL

MTRNR2L6

Nucleotide Accession Number

NM_001190487.2

Frequently Asked Questions

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