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FOXC2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Forkhead box C2

Gene Aliases

FKHL14; forkhead box C2 (MFH-1, mesenchyme forkhead 1) ; forkhead box protein C2; forkhead, Drosophila, homolog-like 14; forkhead-related protein FKHL14; LD; mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH1; MFH-1; MFH-1, mesenchyme forkhead 1; transcription factor FKH-14.

Gene ID

2303

Accession Number

NP_005242.1

Reactivity

Homo sapiens, Human

Target

Forkhead box protein C2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FOXC2 ELISA Kit (Human) (OKEH02234) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FOXC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FOXC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FOXC2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.056 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

54 kDa

Specificity

Natural and recombinant Human Forkhead box protein C2

Protein Length

501

NCBI Gene Symbol

FOXC2

Protein Name

Q99958

Gene Name URL

FOXC2

Nucleotide Accession Number

NM_005251.2

Frequently Asked Questions

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