CYP3A5 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cytochrome P450 family 3 subfamily A member 5
Gene Aliases
Aryl hydrocarbon hydroxylase; CP35; CYPIIIA5; cytochrome P450 3A5; cytochrome P450 HLp2; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; cytochrome P450-PCN3; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450PCN3; PCN3; xenobiotic monooxygenase.
Gene ID
1577
Accession Number
NP_000768.1
Reactivity
Homo sapiens, Human
Target
Cytochrome P450 3A5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CYP3A5 ELISA Kit (Human) (OKEH02233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP3A5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP3A5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP3A5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Human Cytochrome P450 3A5
Protein Length
502
NCBI Gene Symbol
CYP3A5
Protein Name
P20815
Gene Name URL
CYP3A5
Nucleotide Accession Number
NM_000777.4
Frequently Asked Questions
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