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AP2B1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Adaptor related protein complex 2 subunit beta 1

Gene Aliases

Adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta) ; adaptor protein complex AP-2 subunit beta; adaptor related protein complex 2 beta 1 subunit; adaptor-related protein complex 2 subunit beta; ADTB2; AP105B; AP-2 complex subunit beta; AP2-BETA; beta2-adaptin; beta-2-adaptin; beta-adaptin; CLAPB1; clathrin assembly protein complex 2 beta large chain; clathrin-associated/assembly/adaptor protein, large, beta 1; plasma membrane adaptor HA2/AP2 adaptin beta subunit; testicular tissue protein Li 22.

Gene ID

163

Accession Number

NP_001025177.1

Reactivity

Homo sapiens, Human

Target

AP-2 complex subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AP2B1 ELISA Kit (Human) (OKEH02228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP2B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP2B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP2B1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.11 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

105 kDa

Specificity

Natural and recombinant Human AP-2 complex subunit beta

Protein Length

937

NCBI Gene Symbol

AP2B1

Protein Name

P63010

Gene Name URL

AP2B1

Nucleotide Accession Number

NM_001030006.1

Frequently Asked Questions

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