TMPRSS4 ELISA Kit (Mouse)
Product Specifications
Gene Name
Transmembrane protease, serine 4
Gene Aliases
CAP2; channel-activating protease 2; channel-activating protease 4; mCAP2; membrane-type serine protease 2; transmembrane protease serine 4.
Gene ID
214523
Accession Number
NP_663378.1
Reactivity
Mouse, Mus musculus
Target
Transmembrane protease serine 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TMPRSS4 ELISA Kit (Mouse) (OKEH02206) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse TMPRSS4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMPRSS4 and incubated. The TMPRSS4 found in the sample or standards competes with the biotinylated TMPRSS4 for limited binding sites on the immobilized anti-Mouse TMPRSS4 antibody. Excess unbound biotinylated TMPRSS4 and sample or standard TMPRSS4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMPRSS4 captured in the well and inversely proportional to the amount of TMPRSS4 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.786 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 kDa
Specificity
Natural and recombinant Mouse Transmembrane protease serine 4
Protein Length
435
NCBI Gene Symbol
Tmprss4
Protein Name
Q8VCA5
Gene Name URL
Tmprss4
CAS Number
7732-18-5
Nucleotide Accession Number
NM_145403.2
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